PepGel PGmatrix™ 3D Cells – Advanced 3D Cell Culture Platform
Physiologically Relevant 3D Microenvironment: PGmatrix™ 3D Cells provides a powerful, animal-free 3D model, enabling cells to grow and self-organize into true 3D architectural shapes (i.e., round, massive, elongated, branched, polarized cavity, organoids shapes) that closely mimic in vivo heterogenous physiology [1-3]. Our hydrogel system is highly cytocompatible with primary cells and established cell lines, including normal, cancer, and stem cells (hiPSC, MSC, NSC, NPC, HSC), as well as various blood-derived cells.
User-Friendly, Versatile Handling: The well-defined xeno-free PGmatrix™ formulation supports easy cell encapsulation (embedding), plating, and recovery at neutral pH and physiological temperature (room temperature or 37 °C) [3-5]. Cells and organoids can be harvested without exposure to acidic or chilled conditions. The matrix’s self-healing nanofibril network allows direct pipetting for bioprinting or lab-on-chip applications without any post-printing crosslinking [5-6].
High-Content, Predictive Models: PGmatrix™ naturally supports formation of 3D cell structures in domes or sheet geometry. It is ideally suited for laboratory research or commercial high-throughput screening, facilitating uses for accurate drug efficacy and toxicity. Tumor organoids cultured in PGmatrix™ secrete extracellular vesicles (exosomes) whose RNA profiles show ~96 % fidelity to patient bioinformatic data [3].
Flexible Kit Options: Cells are remarkably diverse, and each cell type thrives in its own niche microenvironment. PepGel PGmatrix offers a flexible, tunable formulation to meet the needs of your specific cell types.
At PepGel, we work with you to recommend the most appropriate PGmatrix formulation, whether for single cell types or complex co-cultures. PGmatrix can be supplied:
- Pre-formulated in standard cell culture media
- Combined with your preferred culture medium
- As a fully customized formulation
A typical PGmatrix™ kit includes:
- PGmatrix nanofiber solution – neutral-pH hydrogel precursor
- PGworks trigger solution – initiates rapid formation of the 3D microenvironment
- Your success is our priority. We warmly invite you to request a quote or consult with us for a tailored PGmatrix formula that best fits your application.
Request Quote for PGmatrix 3D Cells Products.
- PG1001-010-S, The most popular matrix for 3D cells, tumor organoids, organ organoids, iPSC (induced pluripotent stem cells), etc cultures.
- PG1002-010-INT2, The best matrix for endothelial cells, neural stem cells, MSCs (mesenchymal stem cells), epithelial cells, MDCKs, etc.
References
- Sun, XS. Qi, GY., PGmatrix™ Enables Physiologically Relevant 3D Tumor Organoids. PepGel PG No. 0001 (2025).
2. Sun, XS. Qi, GY., PGmatrixTM Warrants Lumen Cyst Formation within MDCK Organoids. PepGel PG No. 0003 (2025).
3. Thippabhotla, S., Zhong, C., and He, M., 3D cell culture stimulates the secretion of in vivo like extracellular vesicles, Scientific Reports, (2019) 9:13012 | https://doi.org/10.1038/s41598-019-49671-3.(2019).
4. Xu, J., Qi, GY., Sui, C., Wang, W., Sun, XS. 3D h9e peptide hydrogel: An advanced three-dimensional cell culture system for anticancer prescreening of chemo-preventive phenolic agents. Toxicology in Vitro, 61(2019)104599 (2019).
5. Li, Q., Qi, GY., et al., Universal Peptide Hydrogel for Scalable Physiological Formation and Bioprinting of 3D Spheroids from Human Induced Pluripotent Stem Cells. Advanced Functional Materials, DOI:10.1002/adfm.202104046. (2021).
6. Soltantabar, P. et al., Heart/liver-on-a-chip as a model for the evaluation of cardiotoxicity induced by chemotherapies. Organs-on-a-Chip 3 (2021) 100008. https://doi.org/10.1016/j.ooc.2021.100008 (2021).