PepGel™ PGmatrix™
Stem-X Platfrom

Integrated 3D Platform for Stem Cell Maintenance, Expansion, and Differentiation

PepGel™ PGmatrix™ Stem-X platform delivers a complete solution for stem cell culture, with specialized applicability to hiPSC maintenance, expansion, and differentiation [1,2]. The PGmatrix™ Stem-X platform provides optimized protocols for generating a wide range of somatic cells and organoid models, such as  cerebral, cardiac, and hepatic organoids.

Physiologically Relevant 3D Microenvironment

PGmatrix Stem-X recreates natural 3D niche microenvironments that enable the maintenance, proliferation, and differentiation of multiple stem cell types:

• Human pluripotent stem cells (hPSCs) – embryonic (hESCs) and induced (hiPSCs) 

• Mesenchymal stromal cells (MSCs)
• Neural stem cells (NSCs) and neural progenitor cells (NPCs)
• Hematopoietic stem cells (HSCs)
• hiPSCs derived organoids
• hiPSCs derived cerebral organoids, neural progenitor cell bundles
• hiPSCs derived cardiac organoids
• hiPSCs derived hepatic organoids

Today, nearly 95 % of stem cells are still cultured on flat 2D surfaces, which restricts their differentiation capacity and functional potential. PGmatrix Stem-X overcomes these limitations by allowing cells to adopt their intrinsic 3D morphologies, unlocking their full developmental potential.

Key Advantages

• Simple, scalable workflow – PGmatrix Stem-X is easy to handle in standard laboratory settings and is amenable to scalable flasks and bioreactors.
• Physiological operation – all steps (i.e., cell embedding, plating, and recovery) can be performed at room temperature or 37° C in neutral pH.
• Well-defined and animal-free – PGmatrix Stem-X ensures reproducible, consistent results in a xeno-free microenvironment.
• Versatile formats – PGmatrix-Stem-X is suitable for dome, sheet, or suspension plating.
• Customizable formulations – PGmatrix Stem-X can be tailored to the functional requirements of each stem cell type.
  
  

Because each stem cell type requires a distinct microenvironment, PepGel offers expert guidance. As a result, you are assured of the ideal PGmatrix Stem-X for your specific cell type, applications and workflow.

Request Quote for PGmatrix Stem-X Products.

• PG1003-010-BF, The most popular matrix for 3D hiPSCs maintenance and expansion in embedded dome, sheet or suspension.
• PG1004-010-INT1, The best choice matrix for MSCs, brain organoids cultures, endothelial, epithelial cells.
• PG1005-010-Ncad, the best matrix for HSC (Hematopoietic stem cells), etc.