PGmatrix™ – Frequently Asked Questions (FAQs)
PepGel supports direct B2B institutional and commercial sales, including individual researcher purchases for universities, hospitals, and biotech organizations. If your question is not listed in the FAQ, please contact us at sales@pepgel.com or
1-785-226-6917.
We guarantee a full refund or replacement if nonconforming products are delivered.
Typical in-stock orders are fulfilled within one to seven business days. Customized orders, such as hiPSC-derived cells or organoids, may require one to four months depending on complexity or the extent of customization required.
PepGel seeks to be added as your registered vendor of choice for innovative hydrogel solutions. DUNS, EIN, and W-9 information are available upon request.
We fulfill both bulk and custom formulation orders in the same way. Bulk orders are encouraged and may receive special discount pricing.
A Certificate of Analysis (COA) is provided with all products. Safety Data Sheets (SDS) and Drug Master File (DMF) references are available upon request.
PepGel is based in the USA and ships globally wherever courier services are available.
Yes. Orders may be modified within 48 hours of placement.
Please request a quote online or email us at sales@PepGel.com.
Yes, PepGel rewards repeat customers and referrals with special discounts, as well as considering discounts on selected bulk orders.
Yes. The shear modulus (G') can be tuned from ~50 Pa to 1200 Pa by adjusting PGmatrix concentration. Custom high-strength formulations (1500–5000 Pa) are available upon request.
Typical ranges are 4×10⁴–2×10⁵ cells/mL with gel concentrations from 0.2–1%. Please contact our technical team at sales@pepgel.com or 1-785-226-6917 regarding optimized formulations for your specific cell type.
PGmatrix has physiological neutral pH.
PGmatrix can be handled in a temperature range from room temperature up to 37ºC.
Yes. Since PGmatrix is synthetic and not derived from animals, it is xeno-free and contains no components from animals.
PGmatrix can be tailored to pore sizes ranging from 0.01 µm to 100 µm depending on gel configuration.
Unlike conventional 2D cultures or artificial “3D” aggregates, PepGel’s patented PGmatrix platform provides a genuine physiological microenvironment that faithfully mirrors the body’s own regenerative processes ensuring the preservation of structural and functional cellular integrity.
Depending on cell type, cells can form and maintain stable 3D colonies or organoids for one to four weeks.
Cells, organoids, or spheroids can be easily isolated through mechanical disruption of the gel followed by centrifugation, as described in the PGmatrix User Guide.
For most 3D colonies, organoids, or spheroids (100–300 µm in diameter), treat with Trypsin-EDTA (0.25% trypsin + 0.02% EDTA) or another gentle dissociation reagent (e.g., 1X TrypLE) for 10–30 minutes, depending on the cell type. Gently pipette to aid dissociation and monitor the process under a microscope to determine optimal cell separation. For additional information, consult the PGmatrix User Guide.
PGmatrix can be manually printed or extruded via pipette, extrusion printer, or inkjet bioprinter. For details, refer to the PGmatrix Bioink protocol available upon request. PGmatrix is also compatible with microfluidic devices.
PGmatrix 3D Cells is formulated for a broad range of cell types. PGmatrix Stem‑X is specialized for stem cell maintenance, expansion, and differentiation, especially for hiPSC‑derived cells and organoids.
The PGmatrix system includes a basic PGmatrix formulation and ECM-balanced hybrid versions enriched with specific extracellular matrix ligands, tailored for specialized cell types that rely on interactions with integrin, E-cadherin, or N-cadherin for survival and function.
Cells or organoids can be stained either directly within PGmatrix or after recovery from the gel. Similar procedures are followed as in traditional 2D staining protocols, but with longer incubation and wash times to ensure adequate penetration. For flow cytometry, dissociate 3D colonies, organoids, or spheroids into single cells prior to staining and analysis. A detailed user guide is available from PepGel for additional guidance.
Contact us today to learn more about Pepgel and how it can support your projects.
