PGmatrix hiPSC Kit


• 3D cell culture of hiPSC
• hiPSC encapsulation
• Physiological hiPSC spheroids
• High throughput drug screening
• Bioprinting by pipetting

PGgrow is a supplemental solution for 3D culture of pluripotent stem cells (i.e., iPSCs, PSCs, ESCs) in PGmatrix-hiPSC. PGgrow is optional but highly recommended for hiPSC culturing in PGmatrix. Without PGgrow, It is the user’s responsibility to choose appropriate growth supplement.

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The PepGel hiPSC-qualified PGmatrix (PGmatrix-hiPSC) product is a powerful tool for in vitro 3D human induced pluripotent stem cell (hiPSC) cultures with excellent 10-20 fold proliferation depending on cell types and culture durations at a cell viability of >90%.  PGmatrix-hiPSC produces physiological spheroids (3D colonies) or hiPSCs or similar cell types such as PSCs and ESCs with superior pluripotent biomarkers and high potential differentiation capability.  PGmatrix-hiPSC kit consists of a vial of the PGmatrix-hiPSC patented peptide nanofiber solution, a vial of the PGworks trigger solution, and a vial of PGgrow-hiPSC (PGgrow). The PGmatrix-hiPSC nanofibrils are formulated into a basic or a customer desired cell culture medium in neutral pH. A 3D microenvironment can be formed accordingly for hiPSC spheroid growth. With PGmatrix-hiPSC, cells no longer suffer acidic or chilled conditions. Cultured spheroid colonies are easily harvested from the matrix, and  all operating procedures can be completed at room or body temperature in neutral pH. To view using protocol, click here. To view video for cell encapsulation, as well as spheroid and organoid isolation, click here. To review case studies, click here.